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1、INTERNATIONA1.STANDARDISO4973editionFirst2O23-O9CosmeticsMicrobio1.ogyQua1.itycontro1.ofcu1.turemediaanddi1.uentsusedincosmeticsstandardsCOSmaiqueSMicrobio1.ogieContrd1.equa1.itydesmi1.ieuxdecu1.tureetdesdi1.uantsuti1.isesdansIesnormesre1.ativesauxCosmetiquesISO2023COPYRIGHTPROTECTEDDOCUMENTISO2023I
2、UirhM*hedbdi1.iUedotherwiseupdhi.o啪InyM1.tta0DmkfifiU81.andonnet8CH-1214Vernier,GenevaPhone：M1.22749O1.11觥ftte:丽丽BQrgPub1.ishedinSwitzer1.and10.4.2FOri耳岫耕W柳prpCs13AnnexA(normative)Specificationsforgrowthpromoting,inhibitoryandindicativepropertiesofcu1.turemedia14AnnexB(informative)Qua1.itycontro1.re
3、portforcu1.turemedia.一.19Bib1.iography21Forewordthrough(1.SOmittees.workofPreparingbody1.ntemationa1.Standardsisnorma1.1.ytechnica1.e1.ectrotechnica1.standardization.Internationa1.E1.ectrotechnica1.Commission(IEC)ona1.1.mattersofdescribedtypesofISO/IECDirectivesrShouIdbePartiCU1.ar,documentwasapprov
4、a1.accordancewiththeISO（八）patent(三).attcntion1.akcs1.hcnopossibi1.ityconcerningimp1.ementationvahdityapp1.icabi1.ityinvo1.vec1.aimedcautioncdavai1.ab1.cWWW0iwg/PaUHKdatcstinformation,Whichresponsib1.eidentifyinganypatentconstitutenameendorsement,documentisinformationgivenfortheconvenienceofusersandd
5、oesnotexpressionsre1.ated。伸nization(WTO)assessment,asinwe1.1.asinformationBarrierstoTradeadherenceseeThisdocumentCommittcepreparedStandardizationComnntteeTechnica1.CommitteeCEN/TCco1.1.aborationwithcomp1.eteIistingquestionshodiesthiscandocumentWwwTkiherg三effiberstH)natiQna1.standardsbody.AO2023-A1.1
6、.rightsreservedISO(theInternationa1.OrganizationforStandardization)isawor1.dwidefederationofnationa1.standardsbodiesmemberbodies).TheEachmemberinterestedinsubjectforwhichcarriedoutcommitteehasbeenestab1.ishedhastherighttoberepresentedonthatcommittee.Internationa1.organizations,governmenta1.andnongov
7、ernmenta1.,in1.iaisonwithISO,a1.sotakepartinthework.ISOco1.1.aboratesc1.ose1.ywiththeTheproceduresusedtodeve1.opthisdocumentandthoseintendedforitsfurthermaintenancearedifferentinthedocumentPart1,Innoted.ThiSthediffurectdraftedincriteriaforeditoria1.ru1.esoftheISO/IECDirectives,Part2(seewww.iso.org/d
8、irec1.ives).vofdrawsISOtopositionthatthetheevidence,ofthisordocumentmayofanytheusepatentrightsinrespectthereof.Asofthedateofpub1.icationofthisdocument,ISOhadnotreceivednoticeof（八）PatCnt(三)whichmayberequiredtoimp1.ementthisdocumentHowever,imp1.ementorsaredatabasethatthisatmaythe.ISOsha1.1.behe1.dmayf
9、orobtainedfromtheora1.suchpatentrights.AnytradeusedinthisForanexp1.anationofthevo1.untarynatureOfstandards,themeaningofISOspecifictermsandtheWor1.dTradeconformityprincip1.estheTechnica1.about1.SO,s(TBT)1towww.iso.org/iso/foreword.htm1.theEuropeanwasforbyTechnica1.(CEN)1SOTC217,Cosmetics,in392,Cosmet
10、ics,inaccordancewiththeAgreementontechnica1.cooperationbetweenISOandCEN(ViennaAgreement).Anyfeedbackoroftheseonbefoundatshou1.ddirectedusers.IntroductionThequa1.ityofcu1.turemediaUSedinthecurrentstandardsforcosmeticmicrobio1.ogyisanessentia1.partofmicrobio1.ogica1.ana1.ysisre1.iabi1.ityandneedstobev
11、erified.Checkingdifferentparametersofcu1.turemediasuchasgrowthpromotion,absenceofmicrobia1.growthfornon-inocu1.atedcu1.turemedia,physica1.characteristics,canhe1.ptoassesstheirqua1.ity.ifi1.rrUj期AiMJadVMntemethn1.kgwva次晰Oancequa1.itMiteriathwI坳UgcsueurctbMheticsperformance.Thisapp1.iesto：a) commercia
12、1.1.yready-to-usecu1.turemedia：cu1.turemediab) ingredients.preparedfromdehydratedCU1.tUremediap1.usadditiona1.ingredients,oron1.y一SO4973:2023Cu1.turemediumInternationa1.StandardMicroorganismsPropertiesIncubationtemperatureIncubationtimeon1.TagarmediumISOS21149/Paphy1.ococcusaureusScherichiaco1.iCeud
13、omonasaeruginosarowthpromotion32,52,5)C48h)tatodextroseagarASO11930SPeryHIUSbras1.1.iensis(rowthpromotion22,52,5)S3d(PDA)ibourauddextrose(SO11930andidaa1.bicansCrowthpromotion32,52.5)cC48havmedium(SDA)bourauddextroseOndidaa1.bicansSO162124andidaa1.bicansCrov/thpromotion22,52,5)5dir11ud1.un(SD)bourau
14、ddextroseprmediumwithI1.oramphenico1.SO21322andidaa1.bicans(rowthpromotion22,52,5)5dSO16212(SO21322andidaa1.bicans(Crowthpromotionrowthpromotion252,5)0C2525)。CS3dS3dSDCA),pticsoyagar(TSA)ISOrsoybeancaseinigcstagarmedium(SCDA)S1149iP.taphy1.ococcusaureusScherichiaco1.i(eudomonasaeruginosaandidaa1.bic
15、ansrowthpromotion32,52,5)66hTab1.eA.1.-Enumerationcu1.turemediaForgrowthpromotiontest,theIncubat1.ontimeatthespecifiedtemperatureistheshortestperiodoftimeseciedinthere1.evantInternationa1.Standardoncosmetics.&一SO20231A=Ihsreserved4973:2023(E)Cu1.turemediumInternationa!StandardMicroorganismsPropertie
16、sIncubationtemperatureIncubationtime4gon1.T1.OO,modifiedugon1.Tidotherenrichment)rothsSSO184151Caphy1.ococcusaureusScherichiaco1.iCeudomonasaeruginosaundidaa1.bicansrowthpromotion32,52,5)520hTab1.eA.2Enrichment1.iquidcu1.turemediaForgrowthpromotiontest,theincubationtimeutthesprifirdtemncniturcisthes
17、hortestperidftimesptxi11edinthere1.cv;intInternationa1.Sunchrdoncosmetics.0-SO2023，A=hcsreserved&一SO20231A=IhsreservedCu1.turemediumInternationa1.StandardMicroorganismsPropertiesIncubationtemperatureIncubationtime”1.airdParkerSSO22718aphy1.ococcusaureusCPS1.rowthpromotion(ndicativ4BhrowthPromotion(n
18、dicatfve(CiMwgrCCnpigment(pyvanin).whichf1.uorescesS2,52,5)32,5t2,5)0C24h：4h-4hscherichiaco1.i1.ndcrUV1.ight)IacConkeyIS021150SScher1.ch1.aco1.iC(Ironhibition(32,5t2,5)H8hrowthPromotion(ndicative(rickreourauddextroseISOaarmediumwithI1.oramphenico1.SDCA)(18416andidaa1.bicansC(Vnhibition(3252,5)48hrow
19、thpromotion(ndicative(rhtetobeRe,creamyand32,52,5)32,52,5)24h;4h-48hscherichiaco1.ironvex)Tab1.eA.3Detectioncu1.turemediaInhibktion(32,5t2,5)48h)rgrowthpinmotiontest,theincubationtimeatthespecifiedtemperatureistheshortestperiodoftimespecifiedinthere1.evantInternationa1.Standardon:osmctics.rthetestoi
20、ndicativeProPerdeSJheincubationtimeatthespecifiedtemperatureisthemediumtimespecifiedinthere1.evantInternationa1.Standardon:osrnetics.“thetestOdinhibitionPn1.PertieS,theincubationtimeattheSPRcifiedtemperatureistheIOnXeStPeriOdOfHrneSPedfiedinthere1.evantInternaHonaIStandardoncosmetics.一SO4973:2023Cu1
21、.turemediumIIternationa1.StandardMicroorganismsPropertiesIncubationtemperatureIncubat1.ontimeaCQndidQa1.bicansC1urnmea1.agarwith%po1.ysorbate80SO18416C(1rowthpromotionndkative(arRc,hh1.vrcfracti1.c,32,5i2,5)QC32,52,5)3ds3dScherichiaco1.ithck-wa1.1.edch1.amydospore)足VinReosinethy1.eneb1.ueagarncdiumS
22、O21150CUf1.1叫rowthpromotion(ndicativcIeta1.1.1.csheenunderree1.ed1.ightandb1.ue-b1.ackpearanceundertransmit-3252,5)32,52,5)C424h4h-48heudmonasaeruginosaed1.ight)P*eudomonasPAgarSO22717(rgrosvthrmotontest,theincubationtimeatthespecifiedtemperatureistheshortestperiodoftimespecifiedinthere1.evantIntern
23、ationa1.Standardon4973:2023(E):osmetics.rindicativeorInhibitior0-SO2023，A=hcsreservedproperties,theincubationtimeatthespccifi(xitemperatureisthemediumtimespecifiedinthere1.evantInternationa1.Standardoncosmetics.ProPerHeSjheincubationHmetheSPeCifiRdtemperaturetheIQnReStPeriCdOfHmeSPedf1.edinthere1.ev
24、antInternationa1.StandardCncosmetics.AnnexB(informative)Qua1.itycontro1.reportforcu1.turemediaTab1.eB.1.Examp1.eofcu1.turemediaqua1.itycontro1.reportforcu1.turemediausedincosmeticsmicrobio1.ogystandardsMANUFACTURER,SINSTRJCT1ONSandPREPARINGCONDITIONSCu1.turemediumname:STERIUZAT1.OrCONDITIONSandSTORA
25、GECyc1.etime:Packaging:Storageconditions：PHExpectedva1.ue：Date：Signature：ABSENCEJfmicrobia1.growthExpectedresu1.tsNomicrobia1.轲WthDate:Signature:GROWTHPROMOTIONMicroorganisms&testconditionsDate：Signature:INDICATIVEPROPERTIESMicroorganisms&testconditionsDate:Signature:INHIBITIONPROPERTIESMicroorganis
26、ms&testconditionsIDate:Signature:Bib1.iographyi聘(ftY:iii蜀Og时EremodmedjaanjmQ1.feedandwaterPreparation,production,storageand12fSM1.g30,CosmeticsMicrobio1.ogyEva1.uationoftheantimicrobia1.protectionofacosmetic3ISO16212,CosmeticsMicrobio1.ogyEnumerationofyeastandmou1.d4ISO17516,CosmeticsMicrobio1.ogyMi
27、crobio1.ogica1.1.imits7网910111.12ISO18415,Cosmetics-Microbio1.ogyDetectionofspecifiedandnon-spec1.fiedmicroorganismsISO18416,Cosmetics-Microbio1.ogyDetectionofCandidaa1.bicansISO21149,COSmetiCS-Microbio1.ogyEnumerationanddetectionofaerobicmesophi1.icbacteriaISO21150,CosmeticsMicrobio1.ogyDetectionof
28、Escherichiaco1.iISO21322,CosmeticsMicrobio1.ogyTestingofimpregnatedorcoatedwipesandmasksISO22717,CosmeticsMicrobio1.ogyDetectionOfPseudomonasaeruginosaISO22718,CosmeticsMicrobio1.ogyDetectionofStaphy1.ococcusaureus火6%p邠用熏露芯空、博欷?POgyGuide1.inesfortheriskassessmentandidentificationof13树gSgfi9秘K(fKif，械照fPO/触力耐4州例朋用Wvirucida1.(inc1.udingbacteriophages)activity14JapanesePharmacopeia18thEdition-JP18.15UnitedStatesPharmacopeia,USP44NF39.116EuropeanPharmacopoeia1IthEdition.
