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1、Characterizationofpo1.ykctidcsynthasegenesfromE1.sinoespp.和囊腔MPKS嘱因特性分析目的：得到花生疮痂病的的PKS域因，明确其PKS的结构域，证明由素的合成受到PKS的网控.方法：I使用简并引物对其胡君合iKS保守区进行扩增，通过染色体步格，泅得其全长.2对荻得的全长翻译后进行宽基酸的进化树分析.确定其种类3对基因表达进行研究讨论：Materia1.andmethodsFunga1.strainsandgrowthconditions尾胞(Genebanktiff).柑橘(Genebimk己有，他葡,花生Iso1.ationofthek
2、etovnthase(KS)domainusingdegenerateprimers采用简并引物扩增PKS的KS保守区域.步骤方法Identificationofpartia1.PKSsequencesKS保守区域熨基取序列对比(尾他.柑橘.匍葡,花生.黄曲褥毒索,爆刀菌素)Genomewa1.king获得权因全长，标记各个PKS域分布及与其他相似的比较SAPKSPhy1.ogeneticana1.ysisofPKSsequences进化树选择不同类型的PKS基因类型1.2.3),选择还原鞭及非还原型.(参考2003)选择还原型和非还原型两种.Expressionana1.ysesofPKSg
3、enes测定各个域的表达量及整个基因的衣达量，明确该基因的表达是否与光照有相关性，或者存在其他相关联基因共同调节次生代谢产物.SAPKSEkafage1.gsigtssr1.vtayea1.eesgyvpnrtpstk1.iirigtfygqtsddwreinaaenVdtyfitggvrafakirixyyfkfsgpsfsv,Baaiqi.actsi.wagkdtacagg1.nv1.tnpdifsg1.skgqf1.sktgscktydndadgycrgdgcgsvi1.kryedaiadkdni1.Gci1.gaatnhsaeavsitiiphagaqef1.fkkv1.adagida
4、hqidyvemhgtgtiii (E1.sinocIawocttiiMsnv1.1.1.gdqtadqsp1.1.rk1.v1.rrnda1.vttfheqvsva1.rdevsk1.parqrqdipsf1.k1.n1.vd1.yyQGDKRI.Pi1.estfvaitq1.ghyigyfsenpdr1.pyaantrv1.g1.ctgs1.aatvivsaksise1.vs1.veVriafragacvdaakrv1.qqpgeekepwstivmn1.sekdaqka1.defhsstgtsgpsrayIsaistmavtIsgppsttqk1.fseszxd1.stknrvsipiy
5、apyhaah1.hserd1.qriipdssra1.1.kkyqpht1.1.hssagEC1.VADNTCD1.MSI.AI.VDFI.REPVRWDRM.EESISQ11ATPKAPAKIFTIGVSNVANSMAAA1.KSGQATVsitdhtswivpddynstrgrtqndkiaivgmsgrfpsaasvda1.we1.1.ekg1.dvhrkipaDrfdadahcDpsgksknkshtpygcfidepg1.fdprffnmspreazxqtdpmgr1.a1.vtayezx1.eesgyvpnrtpstk1.hR1.GTFYGQrSDDwREINAAENVDrYFr
6、rGGVRAFAPGRINYYFKFSGPSES1.HHaAiq1.acts1.WGDCDTACAGG1.NV1.TNPDISG1.SKGQ1.SKTGSCKTYDNDADGYCRGDGCGSVI1.KRyedaiadkdnI1.GCI1.GAATNHSAEAVSrrHPHAGNQEF1.FKKV1.AEAGVDAHE1.SYVEMHGTGTQAGDG1.EMTSVTNAFAppnrrrtpdek1.f1.gaikanvgiigeaasgins1.akv1.mmfkknaipanvgikgvmnesfpkd1.gernvhIP1.KQVEFPRNGDAPRK1.F1.NNFSAAGGNTS1.
7、1.1.EDGPVRKPSTVKDPRST1.PITvTARS1.AS1.KRNIDN1.ksyi-Sktpeasits1.sytstarriqhnyriafpatdinkvsea1.daqikdsyspvaitatrvaFCFTGQGsqytg1.gqk1.yqd1.psfkadidq1.nq1.aeshn1.psf1.e1.fdgtdvat1.spvkvq1.gtaciQva1.srmweSwgikpsavighs1.geyaa1.hvagvisasdmvy1.vgrrzxe1.1.vkdctphthgm1.avkasvDairna1.gskMrEMacingpeetv1.cgsseivt
8、aanev匚rsGKSTK1.NVPFAEHSAQVDP1.1.ESFRTIASSVSFKKPAVPV1.SP1.SGD1.ITDVGV1.GPEY1.AKHARETVNrSQA1.ESGQKAK1.FDGKTAW1.E1.GAHPvC1.gmvkgsveTTATAPS.rrgedawkti.ansmcai.h.Agvyinfdeyhrafndaqe1.1.h1.ptysfddkKYw1.dyhnnwt1.TKGEAPyPTKA1.EAAPHiAAAPVERTYKK1.SDSCQKHDEEFSANAGRV1.vQSDVCQPN1.RrvamghqvnDta1.cpss1.yfdmamtvady
9、iyka1.rpsapkigynvzvhmevpkpfiiknvzxppegqh1.q1.eAhad1.eegvAK1.VFRSVTPNGKQ1.VVHAHCTVKFEDINTWQEGwENISFMvKSQ1.EN1.QRKTRTQEAHVigrg1.ayk1.fKVrVNYAKPYRGMEEu1.DNQTTEATASVHFQTKPEDGEFFFPAYWCDSMAH1.GGFIVNATd1.cdsdnyvSvshgwgsikvakemszxnkkyrnyvrmvekpgnvtqgdvyifdgdeiiavvga1.kfqkiPRKV1.NTF1.PPQKAGSAvSApVAPPAAARPA1.
10、KQAPRINTQAQPAKAVPKQVTvAApTPKAAPKKAD1.KKPAgptiitrvmHiiaaetdvdsse1.vdqaafen1.gv)S1.ms1.tis.xrfree1.dmdisst1.td11tvgemkkfFSQYDGEVGtpeqddsdsdsetsgdastpmsevgtpmtipssavsesgkfd1.dtaapasgevsiarqivatemgveiapvavpapsnrRpsspq1.dkvnek1.nasrsadiskypazvtsv1.1.qgnpkizxtkkiff1.pdgsgsatsyvsipniGSDVCAFG1.NCPFMKSPEQWSC
11、GIE1.CAMVY1.KE1.KRRKpTGPYn(iHhAYAVAQA1.1.ANNEEvek1.1.1.1.dSpcpvn1.dp1.p.Xr1.hiffneig1.1.gtgdpsktpkw1.1.phfsaairs1.sdyqpqptikpvktyaIWCREGVAGNPGDPRPPPAEDEDPAPMKW1.1.NHRTDFrDNGWgk1.cgvenmkygvmgghhfsmmKppiivsifdysivDdeanmrqaed1.gq1.ikeg1.sm ICercosporanicotiaweMedgaqmrvvafgdqtydcseavsq1.1.rvrddaivvdf1.e
12、rapav1.kae1.ar1.sseqqeEtprfat1.ae1.VPRYRAGT1.NPAVSQA1.TCIAQ1.G1.FIRQHSSGQEAYPTAHDSC1.TGVCTGA1.；TAVAVGSASSvta1.vp1.A1.HIvavavr1.garaweigsc1.adarrgangryaswIsAVGGiSPQD1.QDRiSAYrAEQA1.ASVSVPY1.SAavgpgqssvsaapvi1.daf1.st1.1.rp1.tttr1.pitzxpyhaph1.ftakdvqhvtdc1.ppsezxWFTVRIP1.ISFSRDEAVSRGASFPAAMSEAVRr)C1.
13、1.RP1.A1.DRMAVSrrNHARD1.GKDSV1.PSP1.A1.SFSDK1.GPQVNSH1.PGAKApTPE1.TSK31PSA1.GAEQQPMAKSP1.A1.1.AASGRFPQSSSMDQFWDV1.INGVDTHe1.vpptrWnaathvsedpkaknvsgtgfgcw1.heagefdaayfnmspreapqvdpaqr1.a1.1.tatea1.eqagvvpnrTsstqknrvgvwygatsndwmetnsaqnvdtyfipggnrafipgrvnyfhkfsgpsytidHMAcNA1.wRGEvDTAivGGTNv1.TNpDMTAG1.D
14、AG1.IF1.SRSGNCKTfDDEADGYCRGEAvvt1.i1.kr1.pdAqadkdpiqasi1.giatnhsaezxzxsitrphagaqqd1.fqqv1.tetg1.tandisvcemhgtgtqagdsgetTSVVET1.AP1.NRSGSAVKnp1.Y1.GAVKSNVGHAESAAGVSS1.AKI1.1.M1.KHSK1.PPHVGIKTK1.NHRI.PD1.aarnthiarsevpwprpkngkrrv1.1.nnfsaaggntc1.v1.edapepedsqevdprehhiva1.sakpdSmvnnijxmitwidkhsgds1.at1.
15、ijQI.SYTTrARRVHHRHRAVATGTD1.1.QiRSS1.QEQ1.DRRVSGERS1PHPPNGPSFV1.AFTGQGSAFAGMGVD1.YKRFASFRSDIARYDQICEGMS1.PSIKAMFEDEKVfstaspt1.Qq1.thvcfqma1.yr1.wks1.gvqakavvghs1.geyaa1.yaagv1.sqsdt1.y1.vgrr1Aq1.Mekh1.sqgtiiam1.avrakeeaivaaidgppgeayefscrxgeqrnv1.ggtvaqiqaakaa1.eakkircqy1.dTPNbXFHTGQVDpi1.pe1.1.qvaaa
16、csiqdpqipvispaygkvirsakdfqpeyfthhcrssvnmvda1.qsaveeg1.1.dknvIG1.EIGPGPVvTQFVKEAVGTrMQTFASINKDKDTWQ1.MTQA1.AKFY1.AGASVEwSRYHEdfpgaqkv1.e1.paygwa1.knyv1.qyVndws1.rkgdpavvVaasn1.e1.sssihkViTNTiTANSDGE1.vVDad1.sredi.hpMvqghqvygvp1.ctpsvyadia1.t1.geyirqvikpgevaqtsvevaemniqsa1.vanntgRVQ1.1.RCAKFDPKAQVAScT
17、FSSIVEQHANCKIRFGS1.EKEKTA1.KSAA1.AAQASMAA1.KTQVGQDDNTYrfskgmiykMIGQ1.ADFDEKYRG1.CA1.T1.DNDAMEASGKVSFKGIPNEGKFHSSRAY1.DA1.SQ1.GGFVMNanegvd1.ekEvfvnhgwgsmrffaa1.dpamtyythvkmtqgkdk1.tgdv1.ifddkqa1.igivggva1.qgvpkr1.mhyiVTAANKKASGPPTEKKTSSPPVEKKASAPVAPTRPAIQRKNAS1.PPPATQvTPQNKTIKTPSVSA1.IAI1.EivsEE1.RMp
18、iDE1.KDDiDFTDAG1.Ds1.1.s1.vissRMRDQ1.GIEFESAQFMEIGSIGG1.KEF1.TR1.SPPVavzwataveivkeea1.ts1.ee1.tdpspneigtvwrda1.ki1.seesg1.tdee1.tddtsfadvgVds1.ms1.vnSr1.rde1.didfpdra1.feecqtifd1.rkrfsgsHiSPDSkpsagdabp1.dssasspPSSEFDGetpmtd1.devfdsppaqkripsppkgrippawsmy1.qgsqkrskei1.f1.fpdgagaatsy1.s1.pr1.gedigvvafn
19、spfmktphkfadhti.pdviasyvegirgrqaqgpyhi.cH11.ayavaqe1.iaageevst1.1.1.iDSPsnG1.DR1.P-RDHCNVG1.EGE1.SR(isiPNKIpew1.mphfrasie1.1.hgYhappmk1.gNktkvmviwageczxfdgvry1Xhippsagdtdedtegmkf1.tekrkdfgatewas1.fpgtDvdarvvesehHFSMMRDSGAQM1.VEHMRDG1.GIVSSspergi1.1.usPafasinCUS1.AAS66004Jpo1.ykeidesynthaseMaqsrq1.f1
20、.fgdqtadfvpk1.rs1.1.svqdspi1.aaf1.dqshyvvraqm1.qsmntvdiik1.artadi.rqmvqKyvdgk1.tpafrta1.vc1.cq1.gchreyeesgnmypqpsdsyv1.gfcmgs1.zvavavscsrs1.se1.1.piavQTV1.iAfk1.g1.ca1.emrdrvdgcsddrgdpwsjiVWG1.DPQQARDQiEVFCRrrNVPQrRrpwiscisknA1.T1.SGSpST1.RAFCAMPQMAQHRTAP1.P1.C1.PAHNGA1.FrQADnTI1.DTTpTTPWEQ1.PGQ!PYI
21、SHVTgnvvqtsnyrd1.ieva1.set1.1.eqvr1.d1.vetg1.pr1.1.qsrqvksvtivpfitrmnetmsNi1.pdsfis1ERD,JGRAIPASGRP(5AGKCK1.AIVSMSGREPESrnEsbWD1.1.YKG1.DVCKEVPRRRWDiNTHVDPSGkarnkgzxtkwgcw1.dfsgdfdprffgispkeapqmdpaqrmzx1.mstyeamerag1.vpDT1.PSrQRDR1.GVFHGvTsNDwMETNTAQN1.DTYFrrGGNRGFIPGRINFCFEFAGPSYTxHHH1.AAIHI.Acns1.
22、wrgDCDTAVAGGTNMIYTPDGHTG1.DKGFF1.SRTGNCKPYDDKADGYCRAEGVGTVF1.KR1.EDA1.ADNDP1.1.GVi1.daktnhszxmsesmtrphvgaqidnmtaa1.nttg1.hpndfsyiemhgtgtqvgdaveMesv1.svfapsETARKADQP1.FVGSAKANVGHGEGVSGVTS1.IKV1.MMMQHDT1.PPHCGIKPGSKINRNFPD1.GRNVHIFepkpwprthtprrv1.innfsaaggnta1.ivedaperhwptekdprsshiva1.szxhvgasmKTN1.ER
23、1.HQY1.1.knphtd1.aq1.sytttarrwhy1.hrvsvtgasveevtrk1.emaiqngdgvsrpkskpKi1.faftgqgsQYATMGKQVYDAYPSFRED1.EKFDR1.AQSHGFPSF1.HVCTSPKGDVEEMAPVvVQ1.A门CI.QMAITN1.MTSFGIRPDVTVGHS1.GEFAA1.YAAGV1.SASDVVY1.VGQRAE1.1.QERCQRGTHM1.AVKAPEA1.SQW!QDHDcevacingpedtv1.sgttknvaevqramtdngikct1.1.k1.pfafhsaqvqpi1.ddffa1.aQ
24、GTFAKPQ1.1.i1.sp1.1.rteiiieqgvvtpsyvaqhcrhwdmaqa1.rsarekg1.iddkt1.vie1.gpkp1.isGmvkmt1.gDkist1.pt1.apnkaiwps1.qki1.tsvytggwdinwkkyhzxpfassqkvvd1.psygwd1.kdyyipyqgdwc1.HRHQQDCKCAAPGHEIKTADYQVPPESTPHRPSK1.DPSKEAFPEIKTTTn.HRVVEETKP1.GT1.VVETdisrkdvng1.argh1.vdgip1.ctpsfyadiamqvgqysmqr1.raghpgagaidg1.vd
25、vsdmvvdka1.vpHGKGPQ1.1.RTT1.TMEWPPKAAATTRSAKvKFATYFADGK1.DTEHASCTVRFTSDAQ1.KS1.RrsvseykthIRQ1.HixiHAKGQFMRYNRKixiYK1.MssMAR1.NPDYM1.1.DY1.V1.NEAENEAASGVDFS1.GssegtfaahpaHVD.1TQVAGFAMNANDNVDIEKQVYVNI1GWDSFQ1YQP1.DNSKSYQVYTKMGQAKEND1.VHGDVVV1.DGEqivaffrgit1.rsvprga1.rvv1.qttvkkadrq1.gfktmpspppptttmpis
26、pykpantqVSSQAIPEATHSHPPQPKHSPVPEAGSAPAAKGVGVSNEK1.DAVMRVVSEESG1.A1.EE1.TDDSNbADMgids1.ssmVigsrfred1.g1.d1.gpefs1.fidcttvrzv1.kdfm1.gsgdagsgsnvedpppsatpginpetdwsssasdsIFASEDHGHSSESGADTGSPPA1.D1.KPYCRPSTSVV1.QG1.PMVARKT1.Fm1.pdgggsafsyas1.pr1.ksdTAVVG1.NCPYARDPENMNCTHGAM1.ESrCNE1.RRRQPRGPYH1.GGWSSGGAF
27、AYVVAEA1.vnqgeevhs1.I1IDPIPQMEQ1.PRFYEHCNSIG1.FTQPGASPDGSTEPPSY1.IPHFTVVDVM1.DYK1.P1.HRRMPKVG1.VWAADTVMDERDAPKMKGMHFMIQKRTEKiP1.XiWD1.MP(iASFDIVRA1.XiANHFt1.mqkehvsiisd1.idrvmaFusariumfujikuroiK1.P19985.1fusarubinc1.ustcr-po1.ykctidcsynthaseMPY1.SAYAPNGITVSGPPQR1.GD1.AHW1.ASKSrrSKAiP1.YSAYHAPH1.YSQK
28、DARRIVDG1.M1.nkavspSEQ1.P1.1.SSTGSKPEERSFAT1.1.EDA1.AQA1.1.HP1.RwGSIH)DVQAA1.ErrGSQQFSVQ$1GSNAEHMYTa1.kkts1.ry1.vpettvpsqptsvpsvpdzvgtskpk1.aivamsgrfpgzxkdneaywd1.1.ykG1.dvhkpvpS1.RwDQQTHvDIyFGAGKNTSATPFGcwi.DdpsefdarffnispreaPqidpaqr1.a1.mtaYEIEQGIVPDArPSTRPDRVG1.FYGvTSNDWMETNSAQNIDTYY1.PGGNRAF1.pG
29、R1.NY1.TKFSGPSYAvAgih1.acna1.wqgdvdtzxiaggtnv1.tnpdyhag1.drghf1.srtgncktfddgadgycRgegvatiiikr1.DDAiAENDpi1.GVV1.tiAYTNHSAESESnRphvgaqrvienki1.neaavdpysvsyvemhGTGTQAGDTemssv1.etfappvaegkvzxrpesqk1.yigsakanighgeaasgvcsvikv1.qm1.kkdtivPhcgiknkiNHRFPTD1.EQRNVR1.AMGPTQWKKGTE1.NPRRVFVNNFSAAGGNSA1.1.1.QDAP
30、PRKQ1.1.ASndsriqfpiAIAKSGVS1.QGNMRSM1.KF1.STNSHVS1.AE1.SYTARRIHHQHRV1.1.P(iATSEEiCSKIETA1.QNNTGVTRPKAAPKVVFTFrGQGAQYPGMGKQ1.FEENEFVRNE1.1.S1.DRIAQN1.GFPSM1.PFIQSDepdvskfapSI.VQ1.ASVC1.Qm.SKI.WASWGrTPTAVVGHS1.GEYAAI.NVAGVI.SDTDTi.f1.vggraq1.1.eqkctrgthAm1.vvkgsqeeitea1.kgqeyetacinspietv1.agpneqiakvke
31、q1.aaasfktt1.1.kvpYafhssq1.ePmvsdiek1.zxgkvtfsdpkipi1.cp1.egtvienanpfnasy1.1Arhsrqpvnm1.ta1.ttayrdgy1.sdrsmV1.EvGPHPAVSGMvKPT1.GQQ1.TCVAS1.QRRRAPwDM1.SAA1.KS1.YDAGAS1.NVVVDYQSNfpgahtvvd1.Payswd1.keywiqyvndws1.rkgdpp1.vinnvsk1.esttihsvveesgdsektgivveadiarkd1.sp1.vQGHEVDGIP1.CTPSVYADIA1.T1.GKY1.1.ERYQ
32、PQQKDDMVvVSDMTVSKAU1.RGDGSRHPIQHAEADWSSQSVS1.KFMSFDNKGN1.QEHSACVV1.卜KDRSHQDA1.QSEA匚11KQKMQN1.RNQirrGESArfnrpmayrM1.Rp1.ARniDDYRA1.DEvv1.NsET1.EAssKisFGTVKRDGDFHTHPAVIDA1.TQSCGFAMNCndhtdidvDVYMNHGWGS1.E1.FEP1.DFEKEYTTYTQMHAGEDKIAvygdvtifdqdrvvaffgqiaiqGvprrv1.kvi1.S1.ESGKKGQpQRQTQDKPRNTPSQTKDSTPKPAQN
33、KPAAKVEPPKFSTA1.RIISEESGIDVSDFTDGTTFAdvgids1.1.g1.tiszxrfqee1.did1.dfna1.ffehptvkd1.rzxf1.gadedvsessssaasdsgrdt11tgsatpfi.qeefaesaeveferai.eiiseesgvarsd1.ddetnfaix(;vds1.i.si.viasrfqdtfg1.NIAHEQ1.MECQTVGD1.KTM1.AREMG1.ATPASKPAAIPPVVSEAVAQETVVTHSDTSN1.AEREQA11E1.VNKYTAGFKAPTSNPNGPP1.GKNESVV1.VTGASGG1
34、.GSH1.VYA1.AQ1.EEVHTVVC1.NRPNREDPTTrqykamrdkgIRIpEH1.KSKvR1.卜QTDTSKPK1.GVADSEYASURSvTH1.IHNAwPMSAKRP1.SG由QFQVFRN1.1.D1.GRecasnrpadfkfsfqmissigvvgqwg1.aagqtgkivvpeerttids1.1.gngyaeakwgcerm1.de1.hKFmRFRPMVVR1.GQ1.AGSKTSGYWNPMEHFGn.IKSSQTI.NAI.PDvrXiN1.NWTPVND1.ADT1.TD1.i1.SDRTPYP1.YH1.DNPiGQQwRDvNNI1.SD1.1.R1.PNKVPFKEw1.DIvrkapqqdnpaa1.1.adf1.edtyi.rmacgG1.v1.dvkhs1.ehsks1.savgpvsetvvrkyihiwkeigf1.kttaedkzxgfeaer1.r1.wgpr
